Synergy LX multi-mode reader

synergy-lxSynergy™ LX Multi-Mode Microplate Reader economically automates many common microplate assays.  The high quality optical  design ensures excellent data in absorbance, fluorescence and luminescence detection modes.  Absorbance optics  include  a xenon  flash lamp and monochromator for continuous wavelength selection from the low UV through the visible range to 999 nm. Fluorescence and luminescence measurements are made with filter- based optics  for optimal specificity and direct  detection to prevent light loss resulting in outstanding accuracy.The broad wavelength ranges enable many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford  and cell viability. Easily accessible filter cubes make running  different  assays quick and effortless,  while the touchscreen user interface simplifies programming. Immediate data display, plus output to a USB flash drive, printer  or Gen5™ Software  makes  the Synergy LX a versatile  assay workstation. An upgradable design allows a lab to buy what is needed today  and add  other detection modes in the future.





  • Affordable multi-mode plate reader
  • Enables many common end point  assays including nucleic acid and protein quantification, ELISA, BCA and Bradford  assay, and cell viability assays.
  • Micro-volume nucleic acid and protein quantification capability with Take3 plates
  • Continuous wavelength selection for UV-Vis measurements; 200 nm to 999 nm in 1 nm increments
  • High performance, high-blocking filters for fluorescence and luminescence
  • Color touchscreen for quick programming and operation and immediate data display
  • Output to USB flash drive, printer or powerful  Gen5 Software



Typical Applications:

  • Fluorescence ELISA
  • Nucleic Acid Quantification (A260 and fluorescence-based)
  • Nucleic Acid Purity Assessment (A260/A280)
  • Gene expression (luminescence and fluorescence)
  • Cell Viability Assays (absorbance MTT, luminescence ATP, various fluorescence-based)
  • Protein Quantification



SLXA Synergy LX with monochromator-based absorbance from 200 nm to 999 nm.
SLXF Synergy LX with filter-based top  fluorescence and luminescence.
SLXFA Synergy LX with monochromator-based absorbance from 200 nm to 999 nm, filter-based top  fluorescence and luminescence.
SLXATS Synergy LX with monochromator-absorbance from 200 nm to 999 nm and  touchscreen interface.
SLXFTS Synergy LX with filter-based top  fluorescence and  luminescence, and  touchscreen interface.
SLXFATS Synergy LX with monochromator-based absorbance from 200 nm to 999 nm, filter-based top  fluorescence and luminescence, and touchscreen interface.

Note: All Synergy LX configurations include  linear, orbital and double-orbital shaking.


Optional Accessories:


  • Take3 Micro-Volume Plates
  • Gen5™ Secure (for 21 CFR Part 11 compliance)
  • Fluorescence Test Plate
  • Absorbance Test Plate
  • Luminescence Test Plate
  • Product Qualification Package















Detection mode:                   UV-Vis absorbance, fluorescence intensity, luminescence

Read  method:                         End point  (onboard software)

End  point, kinetic, area  scanning, absorbance spectral scaning

(under  Gen5 control) Microplate types:  6- to 384-well plates

Other labware:                       Take3 Micro-Volume Plates

Shaking:                                     Linear, orbital, double-orbital

Software:                                   End point  protocols (onboard software)

Full data analysis and  reporting (under  Gen5 control)



Light source:   Xenon flash lamp

Detector: Photodiode

Wavelength selection:   Monochromator

Wavelength range:   200 – 999 nm, in 1 nm increments


Bandwidth: ≤5 nm

Wavelength accuracy:  ±2 nm

Wavelength precision: ±0.2  nm (standard deviation)

Dynamic range:0 to 4.0 OD

Resolution:  0.001  OD (onboard software)

0.0001 OD (under  Gen5 control)

Pathlength correction:                     Yes (under Gen5  control)

Optical density:

Accuracy:     <1% at 2.0 OD

<3%  at 2.5 OD

Linearity:             <1% from 0 to 2.5 OD

Repeatability:     <0.5%  at 2.0 OD

Stray  light:          0.03% at 230 nm

Reading speed (kinetic):   96 wells: 12 seconds

384  wells: 23 seconds


Fluorescence Intensity

Light source:                  Halogen lamp

Detector:                       PMT

Wavelength selection:   Bandpass filters

Wavelength range:      200 – 700 nm (low noise  PMT)

200  – 850 nm (red-shifted PMT)

Dynamic range:            7 decades

Sensitivity:                     Fluorescein 2 pM

Reading speed (kinetic):   96 wells: 24 seconds

384  wells: 76 seconds



Wavelength range:          200 – 700 nm (850 nm option)

Dynamic range:                7 decades

Sensitivity:                        10 amol ATP


Physical Characteristics

Connectivity:  (1) USB 2.0 ports for computer control

(2) USB 2.0 ports for printer connection and  USB thumb drive

(touchscreen configurations only)

Dimensions:            15” H x 15” W x 15” D (with touchscreen)

(38.1  cm H x 38.1 cm W x 38.1 cm D)

12”  H x 15” W x 15” D

(30.5  cm H x 38.1 cm W x 38.1 cm D)

Weight:                     ≤27 lbs (12.3 Kg)

Power:                       External  24VDC power supply  compatible with 100-240 volts AC @50-60Hz. 60W maximum  consumption.












BioTek Instruments, Inc.

Highland  Park, P.O. Box 998

Winooski, Vermont 05404-0998, USA

Phone:  802-655-4040 • Toll-Free: 888-451-5171

Outside  the USA: 802-655-4740