Halotech- specialized kits and reagents

halotech

 

 

 

 

 

 

HalotechDNA, based in Madrid and founded in 2008, is a leading biotechnology company dedicated to research, development and marketing of solutions in the field of fertility. Our in vitro diagnostic kits, together with support for medical professionals, are a reflection of the innovative and high quality effort we bring to our solutions.

FLUOGREEN

fluogreen

The Fluorescence Microscopy Staining Kits reduce background noise and provide  higher contrast images with a DNA-specific fluorochrome for use with Halosperm®, Halosperm G2® and Halomax®.

Available in Green and Red.

 

 

 

 

 

 

fluored

 

 

 

 

 

 

 

 

 

VITALTEST

vitaltesthq

Vitaltest®

Vitaltest® allows the evaluation of the quality of the sperm membrane and the differentiation between live spermatozoa (intact membranes) and dead ones (affected membranes)

Rapid application, reliable, and low-cost technologies are essential for quantifying the state of the cell populations. The viability of spermatozoa in any ejaculate is related to the presence or absence of altered membranes.

Its use is advisable for patients with progressive motility below 40% to evaluate the proportion of live and dead spermatozoa. This test is used as a check on the motility evaluation, as the percentage of dead cells should not exceed that of immotile spermatozoa.

Cases of necrozoospermia are related to epididymal pathology (Wilton et al, 1988; Correa-Pérez et al, 2004). A high percentage of vital but immotile spermatozoa is indicative of structural defects in the flagellum (Chemes and Rawe, 2003).

Applications of Vitaltest®

  • Immediate determination* of the quality of the membrane of spermatozoa in the ejaculate.
  • Determination of necrozoospermia.
  • The incubation of sperm with the reagent is not necessary.

 

OXISPERM

oxisperm-hq

Oxidative stress is essentially an imbalance between the production of free radicals and the body’s ability to counteract their potential negative effects. Blockage of oxidative stress is achieved through the action of antioxidant substances, which the body generates itself or which are administered externally.

Changes in the normal redox state of cells can cause cell toxicity, induced directly by the reactive oxygen, or from its derivatives like superoxide radicals, hydroxyl radicals or peroxides. These types of molecules can generate damage to all cell components–proteins, lipids, and DNA–if not offset by specific antioxidant action.

The human spermatozoon is highly susceptible to oxidative stress. Peroxidative damage directly affects the lipid component of the membrane and also generates breaks in the chains of both nuclear and mitochondrial DNA.

Applications of OxiSperm®

  • Oxisperm®, for its clinical application, is an easy-to-perform and reliable assay that allows the measurement of potential excess superoxide anions present in the ejaculate, with a minimum volume of sperm
  • Determination of oxidation levels in the ejaculate and reanalysis after treatment with antioxidants
  •  Evaluation of the oxidation in each patient to select the best sample that can be presented

HALOSPERM

halosperm-hq

Halosperm is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. Halosperm allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis. Conventional semen analysis only studies spermatozoa concentration, motility and morphology.  It is an incomplete study, as it omits analysis of one of the most important parameters, the integrity of the DNA molecule. 15% of men considered to be infertile present normal sperm analyses, which makes a deeper assessment of the sample important. Halosperm facilitates decision-making for medical professionals regarding which assisted reproduction technique is most appropriate for each couple.

 

 

HALOSPERM G2

halospermg2-hq Halosperm is an in vitro diagnostic kit that allows the measurement of DNA fragmentation in a fast, easy, and reproducible manner, without the need for complex laboratory equipment. Halosperm allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis. Conventional semen analysis only studies spermatozoa concentration, motility and morphology.  It is an incomplete study, as it omits analysis of one of the most important parameters, the integrity of the DNA molecule. 15% of men considered to be infertile present normal sperm analyses, which makes a deeper assessment of the sample important. Halosperm facilitates decision-making for medical professionals regarding which assisted reproduction technique is most appropriate for each couple.