MRC Holland

SALSA MLPA kits for copy number quantification

MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be used to determine if a known mutation is present in the DNA or to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and exclusive manufacturer of MLPA assays, offers more than 400 different MLPA panels used for DNA copy number quantification, mutation identification, and DNA methylation status.

Popular MLPA applications

  • Predisposition to Cancer
    • BRCA1, BRCA2, MLH1, MSH2, MSH6, PMS2, APC, MMR, NF1
  • Neuromuscular Disorders
    • SMA, DMD, CMT1
  • Intellectual Disability
    • microdeletion, PWS/AS, FMR1/AFF2, MECP2, Sotos, WBS, SHOX, aneuploidy
  • Solid Tumours
    • Gliomas, MMR, Neuroblastoma, TP53, Breast
  • Methylation profiling
    • Prader-Willi / Angelman syndrome, Fragile X, Beckwith-Wiedemann Syndrome

Advantages and features of MLPA

  • Examine up to 60 targets per reaction
  • Only 50ng of DNA is required per reaction
  • MLPA detects a short sequence of 60-80 nucleotides
  • Detection of single exon deletions and duplications
  • Detection of targeted point mutations
  • One simple protocol for >400 applications
  • Results available within 24 hours
  • Limited lab equipment required: only a thermocycler and a sequence type electrophoresis system
  • User-friendly, reliable, and free data analysis software, Coffalser.NetTM

Visit the MLPA website www.mrcholland.com to:

  • Search for genes, and chromosomal regions of interest to find all related MLPA products
  • Explore the MLPA procedure to learn how to do MLPA
  • Get MLPA support by viewing education, workshop, and troubleshooting material
  • Stay up to date with current MLPA news