MRC Holland

MRC-Holland logo 3 colors







SALSA MLPA kits for copy number quantification


MLPA® is the gold standard for DNA copy number quantification and is used to study both hereditary disorders and tumours. MLPA can also be used to determine if a known mutation is present in the DNA or to investigate the methylation status of DNA sequences. MRC-Holland, the inventor and exclusive manufacturer of MLPA assays, offers more than 400 different MLPA panels used for DNA copy number quantification, mutation identification, and DNA methylation status.


MLPA kit photo2Popular MLPA applications

  • Predisposition to Cancer
    • BRCA1, BRCA2, MLH1, MSH2, MSH6, PMS2, APC, MMR, NF1
  • Neuromuscular Disorders
    • SMA, DMD, CMT1
  • Intellectual Disability
    • microdeletion, PWS/AS, FMR1/AFF2, MECP2, Sotos, WBS, SHOX, aneuploidy
  • Solid Tumours
    • Gliomas, MMR, Neuroblastoma, TP53, Breast
  • Methylation profiling
    • Prader-Willi / Angelman syndrome, Fragile X, Beckwith-Wiedemann Syndrome

Advantages and features of MLPA

  • Examine up to 60 targets per reaction
  • Only 50ng of DNA is required per reaction
  • MLPA detects a short sequence of 60-80 nucleotides
  • Detection of single exon deletions and duplications
  • Detection of targeted point mutations
  • One simple protocol for >400 applications
  • Results available within 24 hours
  • Limited lab equipment required: only a thermocycler and a sequence type electrophoresis system
  • User-friendly, reliable, and free data analysis software, Coffalser.NetTM




MLPA - protocol 2









Visit the MLPA website to:

  • Search for genes, and chromosomal regions of interest to find all related MLPA products
  • Explore the MLPA procedure to learn how to do MLPA
  • Get MLPA support by viewing education, workshop, and troubleshooting material
  • Stay up to date with current MLPA news