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Media and reagents
FertiCultTM Flushing medium
FertiCultTM Flushing medium is intended for in vitro procedures involving human gametes (sperm and oocytes), including washing of gametes, sperm swim-up procedures, intra-uterine insemination (IUI) of the spermatozoa and sperm injection during intracytoplasmic sperm injection (ICSI). FertiCultTM Flushing medium can also be used for human embryo washing and holding, and for embryo transfer (ET) in the uterine cavity.
Sil-Select PlusTM
Sil-Select PlusTM is a gradient system for semen preparation. The following gradients are available:
- Sil-Select PlusTM Upper layer (45%)
- Sil-Select PlusTM 80%
- Sil-Select PlusTM Lower layer (90%)
The Sil-Select PlusTM system can be used for semen preparation for Intra Uterine Insemination (IUI), In Vitro Fertilization (IVF) and IntraCytoplasmatic Sperm Injection (ICSI).
Sil-Select Plus Sperm Washing/Insemination medium is intended for use in Assisted Reproductive Technologies, more specifically for sperm washing/holding and for the production of density gradients. The medium can be used for IUI.
Sil-Select PlusTM is ready-to-use.
GAINTM medium
GAINTM medium is a single-step cell culture medium for use with human embryos and gametes. GAIN™ medium can be used in all the procedures below:
- For semen washing and intra uterine insemination (IUI).
- For oocytes handling/incubation in preparation of, or during fertilization by in vitro fertilization (IVF)/ intra cytoplasmatic sperm injection (ICSI).
- For embryo culture from day 1 to expanded blastocyst stage.
- For embryo transfer (ET).
GAINTM medium is complete and needs no further additives.
10% PVP in FertiCultTM Flushing medium
10% PVP in FertiCultTM Flushing medium is a viscous medium containing polyvinylpyrrolidone (PVP) used for sperm preparation for Intracytoplasmic Sperm Injection (ICSI). These procedures require the capture of individual sperm cells in a glass pipette for injection into the oocyte and this is facilitated by first immobilizing the sperm by placing them in a viscous medium like 10% PVP in FertiCultTM Flushing medium prior to nicking the tail to immobilize sperm completely.
10% PVP in FertiCultTM Flushing medium is ready-to-use.
FertiCult Aspiration medium
FertiCult™ Aspiration medium is a ready to use formulation used for oocyte aspiration and follicular flushing.
FertiCultTM Flushing medium
FertiCultTM Flushing medium is intended for in vitro procedures involving human gametes (sperm and oocytes), including washing of gametes, sperm swim-up procedures, intra-uterine insemination (IUI) of the spermatozoa and sperm injection during intracytoplasmic sperm injection (ICSI). FertiCultTM Flushing medium can also be used for human embryo washing and holding, and for embryo transfer (ET) in the uterine cavity.
FertiCultTM Flushing medium is ready-to-use
Hyaluronidase in FertiCultTM Flushing medium
Hyaluronidase in FertiCult™ Flushing medium is used in the oocyte denudation process in preparation of intracytoplasmic sperm injection (ICSI) or other Assisted Reproductive Technologies. Hyaluronidase digests the hyaluronic acid between the cumulus cells, which makes it easier to remove the cumulus mechanically.
Hyaluronidase in FertiCult™ Flushing medium is ready-to-use.
FertiCultTM IVF medium
FertiCult™ IVF medium is intended for washing and holding of human oocytes, performing fertilization by In Vitro Fertilization (IVF) or, Intracytoplasmatic Sperm Injection (ICSI) (until 2PN).
FertiCult™ IVF medium is ready-to-us
FertiCultTM Mineral oil / FertiCultTM High Viscosity Oil
FertiCult™ Mineral Oil and FertiCult™ High Viscosity Oil are pre-washed mineral oils for use in In Vitro Fertilization (IVF), Intracytoplasmatic Sperm Injection (ICSI) and related Assisted reproductive technologies (ART).
FertiCult High Viscosity Oil is more viscous and has higher density values compared to FertiCult Mineral Oil. The difference in viscosity (and density) is reflected in the practical use of the product and is dependent on the procedures used by a certain laboratory. In this way, laboratories can choose between both types of oil in order to guarantee the most optimal culture system according to their procedures.
FertiCult™ Mineral Oil and FertiCult™ High Viscosity Oil are ready-to-use.
SpermFreezeTM / SpermFreezeTM SSP
SpermFreezeTM and SpermFreezeTM SSP are media for cryopreservation of human sperm for further use in Assisted Reproductive Technologies. SpermFreezeTM can also be used for the cryopreservation of sperm from testicular biopsies (TESE).
SpermFreezeTM SSP is a more concentrated cryopreservation medium which allows a higher volume of sperm to be cryopreserved:
SpermFreezeTM requires 0.7-1ml medium for 1ml sperm
SpermFreezeTM SSP requires 1ml medium for 3ml sperm
SpermFreezeTM and SpermFreezeTM SSP are ready-to-use
FertiVitTM Cooling / FertiVitTM Warming
FertiVit™ Cooling / FertiVit™ Warming are a set of media for vitrification and warming of human oocytes and embryos (zygote till blastocyst).
FertiVit™ Cooling/FertiVit™ Warming are ready to use.
VitriFreezeTM / VitriThawTM
VitriFreeze™ / VitriThaw™ are a set of media for vitrification and thawing of human embryos (morula till expanded blastocyst stage).
VitriFreeze™ and VitriThaw™ are ready to us
Диагностични материали
Spermac Stain
Sperm morphology analysis is one of the basic semen examinations performed in the diagnosis and management of male infertility. Spermac Stain is an in vitro diagnostic staining kit consisting of a fixative and 3 staining solutions for human spermatozoa. Staining facilitates distinction between morphologically normal and abnormal spermatozoa, and enhances visualization of different parts of the sperm cell (head, acrosome, equatorial region, midpiece, tail).
Spermac Stain is a qualitative, non-automated, diagnostic kit for professional use for the staining of human spermatozoa. The purpose of staining spermatozoa is to facilitate differentiation between morphologically normal and abnormal spermatozoa. The stained smears are assessed under direct bright light and oil immersion (1000x).
SpermMar Test IgA
As sperm does not come into contact with the blood circulation, the male reproductive system contains no antisperm antibodies in normal conditions. However, when the blood-testis barrier is breached, the immune system can detect mature sperm as antigenic and form antisperm antibodies that cause sub- or infertility. Antisperm antibodies belong to two immunological classes: immunoglobulin (Ig)A and IgG antibodies. Antisperm IgA antibodies are clinically associated with immunological infertility, and screening can therefore provide help in assessing the male fertility.
The SpermMar Test IgA is a semi-quantitative, non-automated, diagnostic kit for detecting antisperm antibodies of the IgA class on spermatozoa in human semen. It is a rapid, easy-to-use microscopic test.
The SpermMar Test IgA can be performed on fresh, untreated human semen provided that it contains motile spermatozoa. The SpermMar Test IgA can be used as an aid in the diagnosis and management of male infertility.
HOS Test
The hypo-osmotic swelling test (HOS Test) is an in vitro diagnostic (IVD) for professional use to evaluate the vitality of the spermatozoa in a semen sample. In contrast to dead spermatozoa, living cells have intact cell membranes which allow regulated water transport in hypo-osmotic conditions which will result in swelling or curling of the sperm tail upon incubation in HOST medium.
The HOS Test is a semi-quantitative, non-automated diagnostic test to evaluate the vitality (membrane function) of spermatozoa in a semen sample. The HOS Test may help in assessing the diagnosis and management of male infertility. The HOS Test should not be used for the selection of sperm in ART procedures such as intra-cytoplasmatic sperm injection (ICSI).
VitalScreen
VitalScreen uses eosin-nigrosin staining to assess the percentage of live spermatozoa. The technique is based on the principle that dead cells take up eosin through their cell membrane, and as a result stain red. The nigrosin provides a dark background which makes it easier to evaluate the slides. Sperm vitality should be determined when less than 40% of spermatozoa are motile. In samples with poor motility, it is important to discriminate between immotile dead sperm and immotile live sperm.
VitalScreen is a semi-quantitative, non-automated diagnostic test to evaluate the vitality of spermatozoa in a semen sample by using an eosin-nigrosin staining. VitalScreen may help in assessing the diagnosis and management of male infertility.
LeucoScreen Plus
Most human ejaculates contain leucocytes and the predominant form of leucocytes in human semen are peroxidase-positive granulocytes. Excessive presence of these cells (leucocytospermia) may indicate the existence of reproductive tract infection. Leucocytospermia may also be associated with defects in the semen profile (reduction in sperm motility and DNA integrity, raise of sperm viscosity as well as loss of sperm function as a result of oxidative stress, and/or secretion of cytotoxic cytokines by these white blood cells). Although leucocytospermia is not an absolute indication of infertility, this condition is observed on average in 10 to 20% of all infertile men.
When the threshold of one million peroxidase positive white blood cells per ml ejaculate is exceeded, microbiologic tests should be performed to investigate if there is an accessory gland infection. Assessment of accessory gland markers can provide additional useful information about the proper functioning of the epididymis (EpiScreen Plus, FertiPro NV), seminal vesicles (Fructose Test, FertiPro NV) or prostate. Importantly, the absence of leucocytes does not exclude the possibility of an accessory gland infection.
LeucoScreen Plus is a semi-quantitative, non-automated, histochemical and diagnostic kit for the determination of peroxidase-positive white blood cells in human semen.
EpiScreen Plus
EpiScreen Plus™ can be used to determine the neutral alpha-glucosidase activity in semen (plasma), an enzyme which is mainly secreted by the epididymis. The activity of this enzyme is a reliable marker for epididymis function in patients with (very) low sperm concentration or azoospermic patients, having a normal androgen blood level:
- very low activity indicates a bilateral obstruction between the epididymis and the ejaculatory duct.
- low activity may reflect partial obstruction of the epididymis.
- normal enzyme activity is expected when there is an obstruction above the area in which the enzyme is secreted or in cases of non-obstructive azoospermia (testicular disfunction).
EpiScreen Plus™ is a semi-quantitative, non-automated, photometric and diagnostic kit for detecting neutral alpha-glucosidase in human semen or seminal plasma and may be useful for the diagnosis and the management of male infertility.
Fructose Test
The secretion of the seminal vesicles constitutes the main fraction of the ejaculate. Seminal fructose concentration can be used as a marker of the seminal vesicular function. Low fructose in semen is characteristic for partial or complete ejaculatory duct obstruction, bilateral congenital absence of the vas deference, partial retrograde ejaculation and androgen deficiency.
The Fructose Test is a quantitative, non-automated, photometric and diagnostic kit for the measurement of fructose in human semen or seminal plasma (fresh or frozen). The Fructose Test may help in assessing the diagnosis and management of male infertility as fructose in semen reflects the secretory function of seminal vesicles.